Isolation of Early Neurogenesis Genes with Xenopus cDNA Microarray.
10.5625/lar.2010.26.1.109
- Author:
Saet Byeol YU
1
;
Ok Joo LEE
;
Young Ju PARK
;
Sung Young LEE
;
Seung Hwan LEE
;
Jaeho YOON
;
Yoo Seok HWANG
;
Jong Il KIM
;
Jae Yong LEE
;
Jae Bong PARK
;
Sung Chan KIM
;
Jaebong KIM
Author Information
1. Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Korea. jbkim@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
Xenopus;
BMP inhibition;
neurogenesis;
Affymetrix gene chips
- MeSH:
Bone Morphogenetic Proteins;
Clone Cells;
DNA, Complementary;
Ectoderm;
Embryonic Development;
Embryonic Structures;
Expressed Sequence Tags;
Female;
Neural Plate;
Neurogenesis;
Oligonucleotide Array Sequence Analysis;
Pregnancy;
Up-Regulation;
Vertebrates;
Xenopus
- From:Laboratory Animal Research
2010;26(1):109-115
- CountryRepublic of Korea
- Language:English
-
Abstract:
Neurogenesis is the process that develops neuroectoderm from ectoderm. Bone morphogenetic protein (BMP) inhibition in ectodermal cells is necessary and sufficient for neurogenesis in Xenopus embryos. To isolate genes involved in early neurogenesis, Xenous Affymetrix gene chips representing 14,400 genes were analyzed in early stage of neuroectodermal cells that were produced by inhibition of BMP signaling with overexpression of a dominant-negative receptor. We identified 265 candidate genes including 107 ESTs which were newly expressed during the early neurogenesis by blocking BMP signaling. The candidates of 10 ESTs were selected and examined for upregulation in neuroectoderm. Five EST genes were confirmed to be upregulated in neuroectoderm and examined for time-dependent expression patterns in intact embryos. Two EST genes were cloned and identified as a homology of CYP26c (Xl.1946.1.A1_at) and Kielin containing VWC domain (Xl.15853.1.A1_at). One of them, CYP26c, was further characterized for its transcriptional regulation and role of anterior-posterior patterning during neurogenesis. Taken together, we analyzed and characterized genes expressed in early neurogenesis. The results suggest that neurogenesis by inhibition of BMP provides useful system to isolate genes involved in early events of neurogenesis during early vertebrate embryogenesis.
