Effect of porcine bone morphogenetic protein on healing of bone defect in the rabbit radius.
- Author:
Nam Hyun KIM
1
;
kyu Hyun YANG
;
Hwan Mo LEE
;
Sang Hwan OH
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Bone morphogenetic protein; porcine; purification; bone defect; radius; rabbit
- MeSH: Animal; Bone Morphogenetic Proteins; Growth Substances/*pharmacology; Osteogenesis/*drug effects; Proteins/*pharmacology; Rabbits; Radius/drug effects/pathology/radiography; Support, Non-U.S. Gov't; Swine
- From:Yonsei Medical Journal 1992;33(1):54-63
- CountryRepublic of Korea
- Language:English
- Abstract: Segmental long bone defects due to infection or trauma is a difficult problem to manage in patients. We studied the effect of porcine bone morphogenetic protein (pBMP) on healing of defects in the rabbit radius. Porcine BMP was separated and purified from the tibia and femur of pigs by repeated solubilization and precipitation of the protein with different concentrations of urea and GuHCl. The osteoinductive activity of pBMP was confirmed by bioassay using No. 615 mice. In rabbits, about a 15 mm length of radii were removed and 20 mg of pBMP was implanted in the defected area with fibrin sealant (FS), while only FS was implanted in controls. Union of the affected area was observed in 6 weeks in the experimental side. There was no definite evidence of bone bridging across the affected area in the controls. This suggests that pBMP has a bone forming activity in other species and the clinical use of pBMP in treating patients with segmental bone defects is promising.
