The Role of Keratinocyte-derived Chemokine in Hemorrhage-induced Acute Lung Injury in Mice.
10.3346/jkms.2009.24.5.775
- Author:
Byoung Hoon LEE
1
;
Tae Jin LEE
;
Jae Woo JUNG
;
Dong Jin OH
;
Jae Chol CHOI
;
Jong Wook SHIN
;
In Won PARK
;
Byoung Whui CHOI
;
Jae Yeol KIM
Author Information
1. Department of Internal Medicine, Chung-Ang University College of Medicine, Seoul, Korea. jykimmd@cau.ac.kr
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Acute Lung Injury;
LPS;
Hemorrhage;
Keratinocyte-derived Chemokines
- MeSH:
Acute Lung Injury/etiology/*metabolism;
Animals;
Antibodies/immunology/metabolism;
Chemokine CXCL2/analysis;
Chemokines/analysis/blood/*physiology;
Chickens;
HMGB1 Protein/metabolism;
Humans;
Interleukin-1beta/analysis;
Lipopolysaccharides/toxicity;
Mice;
Mice, Inbred BALB C;
NF-kappa B/metabolism;
Neutrophils/immunology/metabolism;
Peroxidase/analysis;
Shock, Hemorrhagic/*complications;
Time Factors;
Tumor Necrosis Factor-alpha/analysis
- From:Journal of Korean Medical Science
2009;24(5):775-781
- CountryRepublic of Korea
- Language:English
-
Abstract:
Dominant inflammatory cytokines might be different depending on the underlying causes of acute lung injury (ALI). The role of kertinocyte-derived chemokine (KC), a potent chemoattractant for neutrophils, has not been clearly established in hemorrhage-induced ALI. In this study, lung injury and cytokine expressison were evaluated in LPS- or hemorrhage-induced ALI models of BALB/c mice. The myeloperoxidase activities at 4 hr after hemorrhage and LPS-injection were 47.4+/-13.0 and 56.5+/-16.4 U/g, respectively. NF-kappa B activity peaked at 4 hr after hemorrhage, which was suppressed to the control level by anti-high mobility group B1 (HMGB1) antibody. Lung expressions of TNF-alpha, MIP-2, and IL-1beta were increased by LPS injection. However, there was only a minimal increase in IL-1beta and no expressions of TNF-alpha or MIP-2 in hemorrhage-induced ALI. In contrast, lung KC increased significantly at 4 hr after hemorrhage compared to control levels (83.1+/-12.3 vs. 14.2+/-1.6 pg/mL/mg by ELISA) (P<0.05). By immunohistochemical staining, lung neutrophils stained positive for KC. Increased KC was also observed in bronchoalveolar lavage fluid and plasma. KC plays an important role in hemorrhage-induced ALI.
