Induction of Angiogenic Cytokines in Cultured RPE by Oxidative Stress.
- Author:
Keun Oh KIM
1
;
Tae Jung YOON
;
Gwang Ju CHOI
Author Information
1. Department of Ophthalmology, Chosun University, College of Medicine, Gwangju, Korea. gjchoi@mail.chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Angiogenic cytokine;
Antioxidant;
Oxidative stress;
Paraquat;
RPE
- MeSH:
Acetylcysteine;
Antioxidants;
Cytokines*;
Interleukin-8;
Macular Degeneration;
Monocytes;
Oxidative Stress*;
Paraquat;
Reverse Transcriptase Polymerase Chain Reaction
- From:Journal of the Korean Ophthalmological Society
2004;45(10):1742-1749
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was undertaken to investigate whether expression of angiogenic cytokines including IL-8 and MCP-1 is induced in the cultured RPE by oxidative stress. METHODS: ARPE-19 cells were exposed to H2O2 and paraquat for 24, 48, and 72 hours and then assayed for the detection of IL-8 and monocyte chemotactic protein (MCP)-1 expression by reverse transcriptase polymerase chain reaction (RT-PCR) method. To determine whether the antioxidant overcame the oxidative stress, we observed expression of IL-8 and MCP-1 gene after ARPE cells were treated by paraquat and antioxidants including N-acetylcysteine (NAC) and pyrrolidine dithiocarbamate (PDTC) at the same time for 72hr. RESULTS: Expressions of the angiogenic cytokines, IL-8 and MCP-1 were increased by incubating with paraquat for 72hr. Antioxidants including NAC and PDTC decreased the expression of IL-8 and MCP-1 gene by counteracting the paraquat effect. CONCLUSIONS: Oxidative stress stimulates angiogenic cytokines including IL-8 and MCP-1 in the cultured RPE. These results may be significant for understanding the cause of age-related macular degeneration and its treatment.
