Dysregulation of MicroRNA-196b-5p and MicroRNA-375 in Gastric Cancer.
10.5230/jgc.2016.16.4.221
- Author:
Seung Woo LEE
1
;
Ki Cheol PARK
;
Jeong Goo KIM
;
Sung Jin MOON
;
Sang Bum KANG
;
Dong Soo LEE
;
Hae Joung SUL
;
Jeong Seon JI
;
Hyun Yong JEONG
Author Information
1. Division of Gastroenterology, Department of Internal Medicine, Daejeon St. Mary's Hospital, School of Medicine, The Catholic University of Korea, Daejeon, Korea.
- Publication Type:Original Article
- Keywords:
MicroRNAs;
Stomach;
Neoplasms;
Microarray analysis;
Polymerase chain reaction
- MeSH:
Apoptosis;
Biomarkers;
Microarray Analysis;
MicroRNAs;
Polymerase Chain Reaction;
RNA;
Stomach;
Stomach Neoplasms*
- From:Journal of Gastric Cancer
2016;16(4):221-229
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Dysregulated microRNAs (miRNAs) can contribute to cancer development by leading to abnormal proliferation of cells, apoptosis, and differentiation. Although several miRNAs that are related to gastric cancer have been identified, the reported results have been inconsistent. The aim of this study was to determine miRNA expression profiles and validate miRNAs up- and down-regulated in gastric cancer. MATERIALS AND METHODS: We evaluated 34 primary gastric cancer tissues and paired adjacent nontumorous gastric tissues. Total RNA was extracted, and low-molecular-weight RNAs (<200 nucleotides) were isolated for further analysis. Two pairs of tissues were processed for GeneChip microarray analysis, and the identified up- and down-regulated miRNAs were validated by real-time quantitative polymerase chain reaction (qPCR). RESULTS: In the set of differentially expressed miRNAs, 5 were overexpressed by more than 2 fold, and 5 were reduced by 2 fold or less in gastric cancer tissues compared with normal gastric tissues. Four of these miRNAs (miR-196b-5p, miR-375, miR-483-5p, and miR-486-5p) were then validated by qPCR, and the relative expression levels of 2 miRNAs (miR-196b-5p and miR-375) were significantly different between cancer and normal tissues. CONCLUSIONS: Our results revealed that the expression of miR-196b-5p and miR-375 significantly correlates with gastric cancer. These miRNAs could therefore serve as diagnostic biomarkers of gastric cancer.
