Production of Recombinant Buckwheat Allergen.
- Author:
Ki Young LEE
1
;
Byeung Ju JEOUNG
;
Jeong Woo RYU
;
Hae Yung YUM
;
Kyu Earn KIM
Author Information
1. Department of Pediatrics, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Buckwheat;
in vitro translation;
Recombinant allergen
- MeSH:
Allergens;
Electrophoresis, Polyacrylamide Gel;
Fagopyrum*;
Hypersensitivity;
Korea;
Molecular Weight;
RNA;
RNA, Messenger
- From:Pediatric Allergy and Respiratory Disease
1998;8(2):215-220
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Buckwheat is one of the most common food allegen in Korea and frequently elicit severe allergic reactions. However, up to now, only few reports on buckwheat allergens have been reproted. The purpose of this study was to isolate mRNA for the production of recombinant buckwheat allergens. METHODS: After the isolation of mRNA from ripening buckwheat seeds, in vitro translation was performed. The proteim patters of in vitro traslate products were identified using SDS-PAGE. RESULTS: A260/A260 ratio total RNA was 1.9 and mRNA was 2.0. In vitro translate products showed towered molecular buckwheat proteins such as 1, 3, and 14kD, while other high molecular weight protein of buckwheat seed were not shown in SDS-PAGE. CONCLUSION: mRNA of buckwheat was purified only from ripening seeds. The composition of mRNA was different according to the ripening periods. It is believed that this finding can give a clue to the basic research of buckwheat allergen.
