Amyloid-beta oligomers regulate the properties of human neural stem cells through GSK-3beta signaling.
- Author:
Il Shin LEE
1
;
Kwangsoo JUNG
;
Il Sun KIM
;
Kook In PARK
Author Information
1. Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Korea. kipark@yuhs.ac
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
amyloid-beta oligomers;
differentiation;
glycogen synthase kinase-3beta;
human neural stem cells;
migration;
proliferation
- MeSH:
Amyloid beta-Peptides/*pharmacology;
Animals;
Apoptosis;
Cell Aging;
Cell Movement;
Cell Proliferation;
Culture Media, Conditioned/chemistry/pharmacology;
Fetus/cytology;
Glycogen Synthase Kinase 3/*metabolism;
HEK293 Cells;
Humans;
Mice;
Mice, Inbred C57BL;
Neural Stem Cells/*drug effects/metabolism/physiology;
Signal Transduction;
Telomere Shortening
- From:Experimental & Molecular Medicine
2013;45(11):e60-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Alzheimer's disease (AD) is the most common cause of age-related dementia. The neuropathological hallmarks of AD include extracellular deposition of amyloid-beta peptides and neurofibrillary tangles that lead to intracellular hyperphosphorylated tau in the brain. Soluble amyloid-beta oligomers are the primary pathogenic factor leading to cognitive impairment in AD. Neural stem cells (NSCs) are able to self-renew and give rise to multiple neural cell lineages in both developing and adult central nervous systems. To explore the relationship between AD-related pathology and the behaviors of NSCs that enable neuroregeneration, a number of studies have used animal and in vitro models to investigate the role of amyloid-beta on NSCs derived from various brain regions at different developmental stages. However, the Abeta effects on NSCs remain poorly understood because of conflicting results. To investigate the effects of amyloid-beta oligomers on human NSCs, we established amyloid precursor protein Swedish mutant-expressing cells and identified cell-derived amyloid-beta oligomers in the culture media. Human NSCs were isolated from an aborted fetal telencephalon at 13 weeks of gestation and expanded in culture as neurospheres. Human NSCs exposure to cell-derived amyloid-beta oligomers decreased dividing potential resulting from senescence through telomere attrition, impaired neurogenesis and promoted gliogenesis, and attenuated mobility. These amyloid-beta oligomers modulated the proliferation, differentiation and migration patterns of human NSCs via a glycogen synthase kinase-3beta-mediated signaling pathway. These findings contribute to the development of human NSC-based therapy for AD by elucidating the effects of Abeta oligomers on human NSCs.
