Application of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Imaging Mass Spectrometry (MALDI-TOF IMS) for Premalignant Gastrointestinal Lesions.
- Author:
Kwang Hyun KO
1
;
Chang Il KWON
;
So Hye PARK
;
Na Young HAN
;
Hoo Keun LEE
;
Eun Hee KIM
;
Ki Baik HAHM
Author Information
1. Digestive Disease Center, CHA Bundang Medical Center, CHA University, Seongnam, Korea. hahmkb@cha.ac.kr
- Publication Type:Review
- Keywords:
Spectrometry, mass, matrix-assisted laser desorption-ionization;
Imaging mass spectrometer;
Biological markers;
Premalignant gastrointestinal lesions;
Chemoprevention
- MeSH:
Biopsy;
Chemoprevention;
Diagnosis;
Mass Spectrometry*;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Biomarkers
- From:Clinical Endoscopy
2013;46(6):611-619
- CountryRepublic of Korea
- Language:English
-
Abstract:
Imaging mass spectrometry (IMS) is currently receiving large attention from the mass spectrometric community, although its use is not yet well known in the clinic. As matrix-assisted laser desorption/ionization time-of-flight (MALDI)-IMS can show the biomolecular changes in cells as well as tissues, it can be an ideal tool for biomedical diagnostics as well as the molecular diagnosis of clinical specimens, especially aimed at the prompt detection of premalignant lesions much earlier before overt mass formation, or for obtaining histologic clues from endoscopic biopsy. Besides its use for pathologic diagnosis, MALDI-IMS is also a powerful tool for the detection and localization of drugs, proteins, and lipids in tissue. Measurement of parameters that define and control the implications, challenges, and opportunities associated with the application of IMS to biomedical tissue studies might be feasible through a deep understanding of mass spectrometry. In this focused review series, new insights into the molecular processes relevant to IMS as well as other field applications are introduced.
