Effects of t-butyl hydrogen peroxide on single SR calcium release channels.
- Author:
Jung Hoon SHIN
1
;
Kwang Ho KIM
;
Chang Kook SUH
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Lipid bilayer; canine latissimus dorsi muscle; SR calcium release channel; t-butyl hydrogen peroxide
- MeSH: Animal; Calcium Channels/drug effects*; Dogs; Hydrogen Peroxide/pharmacology; Peroxides/pharmacology*; Sarcoplasmic Reticulum/metabolism; Sarcoplasmic Reticulum/drug effects*; tert-Butylhydroperoxide
- From:Yonsei Medical Journal 1998;39(1):56-60
- CountryRepublic of Korea
- Language:English
- Abstract: Using lipid bilayer reconstitution technique, we investigated the oxidation effect of t-butyl hydrogen peroxide (tBHP) on the single channel activity of the sarcoplasmic reticulum (SR) calcium release channels isolated from canine latissimus dorsi muscles. When 0.7% tBHP was added in the cytosolic side, the channel activity became suppressed (n = 7), and it was recovered by changing the solution to the control solution. The suppression was due to the change in the gating mode of the channel: before tBHP the channel opened to four sub-conductance levels, but it opened to only one level after tBHP. These effects by tBHP were different from the previous finding using hydrogen peroxide (H2O2), which may be explained by different oxidation patterns between the two oxidants.
