Apoptosis in Experimental Autoimmune Uveitis.
- Author:
Hyeong Gon YU
1
;
Wang Jae LEE
;
Dong Hoon SHIN
;
Young Suk YU
;
Hum CHUNG
Author Information
1. Department of Ophthalmology, Seoul National University, College of Medicine, Korea.
- Publication Type:Original Article
- Keywords:
Experimental uveitis;
Fas ligand
- MeSH:
Animals;
Apoptosis*;
Blotting, Western;
Choroid;
Ciliary Body;
Cornea;
Fas Ligand Protein;
Immunization;
Immunohistochemistry;
In Situ Hybridization;
Inflammation;
Iris;
Rats;
Retina;
Uveitis*
- From:Journal of the Korean Ophthalmological Society
2002;43(3):608-614
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Fas ligand is known to play a key role in intraocular inflammation. We investigated the change of the expression of Fas ligand in the eye with experimental melanin-induced uveitis (EMIU). METHODS: EMIU was induced in 16 Lewis rats after immunization with melanin-associated antigen. Immunohistochemistry, in situ hybridization, and Western blot for Fas ligand were performed serially after immunization. RESULTS: The expression of Fas ligand was higher in the inflammatory eyes at cornea, iris, ciliary body, choroids and retina compared with control eyes. The expression of FasL remained elevated ever at 30 days after immunization in comparison with control eyes even though the inflammation of the eyes had subsided. CONCLUSIONS: These results suggest that the spontaneous regression of intraocular inflammation may be mediated by Fas ligand in EMIU.
