Age-related regional difference of interleukin-1 expression in rat brain after lipopolysaccharide treatment.
10.3346/jkms.2001.16.1.103
- Author:
Gi Yeong HUH
1
;
Mee Sook ROH
;
Hae Rahn BAE
Author Information
1. Department of Pathology, Dong-A University College of Medicine, Pusan, Korea. gyhuh@daunet.donga.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Interleukin-1;
Immunohistochemistry;
Lipopolysaccharides;
Aging;
Rats;
Brain
- MeSH:
Age Factors;
Animal;
Brain Chemistry/drug effects*;
Gene Expression Regulation/drug effects;
Immunohistochemistry;
Interleukin-1/genetics;
Interleukin-1/analysis*;
Lipopolysaccharides/toxicity*;
Male;
Rats;
Rats, Sprague-Dawley;
Receptors, Interleukin-1/analysis
- From:Journal of Korean Medical Science
2001;16(1):103-107
- CountryRepublic of Korea
- Language:English
-
Abstract:
Aging is associated with altered immune responses including dysregulation of cytokine production. Of cytokines, interleukin-1 (IL-1) family has been primarily involved with central nervous system. To evaluate the age-related different response of IL-1 family following peripheral administration of lipopolysaccharide (LPS), immunohistochemical study of IL-1beta and IL-1 receptor expression was performed on Sprague-Dawley rat brain. Experimental animals were divided into four groups; saline-treated young (3-5 months) and old (over 24 months), and LPS-treated young and old groups. After intraperitoneal (i.p.) injection of LPS, three to five rats within each group were killed at 1, 2, 4, 8 and 16 hr. After fixation in 4% neutral buffered formalin, the brain slices were paraffin-embedded. Immunohistochemical staining using labelled streptavidin biotin was performed. The results showed that IL-1beta immunoreactivity was seen in the endothelial cell of pons in both LPS-reated young and old rats, with slightly longer persistency in old group. IL-1RI immunoreactivity appeared initially in the neurons of cerebral cortex in LPS-treated old group, compared with predominantly the cerebellum in LPS-treated young group. In conclusion, our study shows that there is age-related, different neuronal localization of IL-1RI expression at different points of time after LPS treatment.
