Culture of Bovine Retinal Pigment Epithelium: Topographical Differences of Morphology and Growth Rate in Vitro.
- Author:
Soo Won KIM
1
;
Hye Bin YIM
;
Warne HUH
Author Information
1. Department of Ophthalmology, Kangnam St. Mary`s Hospital, The Catholic University Medical College.
- Publication Type:In Vitro ; Original Article
- Keywords:
Age-related macular degeneration;
RPE(Retinal Pigment Epithelium);
Topographical differences)
- MeSH:
Adult;
Cell Culture Techniques;
Humans;
Macular Degeneration;
Retinal Pigment Epithelium*;
Retinaldehyde*;
Serial Passage
- From:Journal of the Korean Ophthalmological Society
1998;39(11):2673-2678
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Cell cultures of adults bovine retinal pigment epithelium(RPE) were propagated from central and peripheral regions of the same eyes to study the topographical differences in cell growth and to compare the differences in growth rate between two areas. The results obtained were as follows: A regional variation in the morpholgy was observed between the RPE from central and that from peripheral regions. Retinal pigment epithelium from central region attached to culture dish more slowly(average 4 days) than those from peripheral region(average 3.5 days) The growth rate of retinal pigment epithelium declined with serial passage in culture. The growth rate of retinal pigment epithelium from peripheral region at the first generation was highest. And there was a statistical difference in growth rate with passing in generation(P<0.05). This study reveals that growth rate and cell activity of RPE from central region are lower than from peripheral region.
