Inhibitory Effect of alpha-Tocopherol on Fibroblast Proliferation in Glaucoma Filtration Surgery with Rabbits.
- Author:
Tae Yoon LA
1
;
Nam Ho BAEK
Author Information
1. Departmento of Ophthalmology, college of Medicine, The Catholic University of Korea.
- Publication Type:Original Article
- Keywords:
alpha-Tocopherol;
Fibroblast prolifeartion;
Glaucoma filtration surgery;
Mitomycin C
- MeSH:
alpha-Tocopherol*;
Blister;
Cicatrix;
Ethanol;
Fibroblasts*;
Filtering Surgery*;
Filtration*;
Glaucoma*;
Lymphocytes;
Mitomycin;
Rabbits*;
Reticulin;
Tocopherols
- From:Journal of the Korean Ophthalmological Society
1998;39(11):2709-2722
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Generally, the postoperative failure of glaucoma filtrationsurgery(GFS) is caused by scarring of filtering site. Mitomycin C(MMC) has been commonly used for preventing the scarring of filtering site. Though the antifibroblastic effect of this drug is approved clinically, its cytotoxic side effect cannot be overlooked,. alpha-tocopherol is known to inhibit proliferation of fibroblast without cytotoxic complications. In this study, we used alpha-tocopherol in GFS of rabbits and investigated the maintenance of bleb formation, complications and inhibition of fibroblast proliferation compared with MMC. Thirty six rabbits were divided into 6 groups Negative control groups were the balanced salt solution soaked group and the 19% ethanol soaked group and positive control group was soaked with 0.2mg/ml MMC during GFS. As experimental groups, group I, II, III were soaked with 100micrometer, 1mM and 10mM alpha-tocopherol. The results were as follows: For the maintenance of bleb formation, at postoperative 2 weeks, all extinguished in negative control groups and each experimental groups from I to III showed 16.7%, 33.3%, 50%, with highest rate of 66.7% in MMC soaked group, but at 2 months, group III and MMC soaked group showed 33.3% equally. Various complications were present in MMC soaked group but rare in alpha-tocopherol soaked groups. On histologic examinations, proliferation of fibroblast and infiltration of lymphocyte in group II, III and MMC soaked group except group I were much less than those of negative control groups and the degree of inhibition was proportional to concentration of tocopherol. There was no statistic difference(P>0.05) in area of reticulin fibers between group III and MMC soaked group and antifibroblastic effects were also similar between these two groups. These result suggest when directlyconcentration(1mM<) as much as MMC. Moreover, it is expected to be used more safely during GFS withous serious cytotoxic complications. However, application method, dosageand possible long term complications.
