Proteomic Approach for Identifying Marker Proteins in the Urinary Bladder Precancerous Conditions Induced by BBN(N-butyl-N-(4-hydroxybutyl) nitrosamine).
- Author:
Hyung Jee KIM
1
;
Wook Young YOUN
;
Na Hae MYONG
;
Cheol Ho HWANG
;
Jin Woo RYU
;
Young Sun LEE
Author Information
1. Proteomics Research Group, Department of Urology, College of Medicine, Dankook University, Cheonan, Korea.
- Publication Type:Original Article
- Keywords:
Proteomics;
Bladder;
Precancerous conditions;
Cytokeratin 20
- MeSH:
Actins;
Animals;
Carcinogenesis;
Carcinoma, Transitional Cell;
Diagnosis;
Drinking Water;
Electrophoresis, Gel, Two-Dimensional;
Follow-Up Studies;
Hot Temperature;
Humans;
Hyperplasia;
Keratin-20;
Mass Spectrometry;
Models, Animal;
Precancerous Conditions*;
Proteomics;
Rats;
Seminal Vesicles;
Shock;
Transcription Factors;
Urinary Bladder Neoplasms;
Urinary Bladder*;
Urologic Diseases
- From:Korean Journal of Urology
2004;45(1):77-83
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Simple and noninvasive methods for the diagnosis of transitional cell carcinoma of the bladder are needed for the prevention of invasive tumor. A proteomic technology has recently been developed to facilitate protein profiling of biological mixtures. We investigated the role of this proteomic approach as a possible tool to detect the marker protein during the initiation stages on BBN-induced bladder carcinogenesis in rats. MATERIALS AND METHODS: Ten rats group A were given 0.05% BBN in drinking water for 12 weeks. Ten rats in group B were designated as a control group and were not given BBN. Whole urinary bladders of all rats were excised at 12 weeks from the beginning of the experiment. Conventional proteomics was performed with high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry. RESULTS: A comparison of urinary bladder hyperplasia tissue with control tissue showed that five proteins; actin gamma2 propeptide, cytokeratin-20, proapolipoprotein, alpha2 actin(alpha-cardiac actin) and heat shock 27kDa protein 1 were over-expressed in hyperplastic tissues. Three protein; transcription factors, seminal vesicle secretory protein VI precursor and hypothetical protein RMT-7 were under-expressed in hyperplastic tissues. CONCLUSIONS: In an animal model system, BBN-induced, urinary bladder mucosal hyperplasia resulted in an increase in five proteins and a decrease in three proteins. Of these altered proteins, CK-20 and SVS-VI seem to be important. The proteomic approach may be a simple and noninvasive method for monitoring and follow-up of bladder cancer patients. However more information is needed regarding CK-20 expression in nonmalignant urological disease and in human tumor tissue, and regarding SVS-VI expression in other organs, for clinical usage.
