Comparison of Various Methods for Detection of Methicillin-Resistant Staphylococcus aureus.
- Author:
Kyung LEE
1
;
Se Ran HEO
;
Soon He CHOI
;
Sang Hoon SONG
;
Kyoung Un PARK
;
Junghan SONG
;
Eui Chong KIM
Author Information
1. Department of Laboratory Medicine, Seoul National University Bundang Hospital, Gyeonggi-do, Korea. m91w95@dreamwiz.com
- Publication Type:Original Article
- Keywords:
MRSA;
Cefoxitin;
Moxalactam;
Oxacillin;
Disk diffusion methods
- MeSH:
Anti-Bacterial Agents;
Cefoxitin;
Diffusion;
Latex Fixation Tests;
Mass Screening;
Methicillin;
Methicillin Resistance*;
Methicillin-Resistant Staphylococcus aureus*;
Moxalactam;
Oxacillin;
Polymerase Chain Reaction;
Sensitivity and Specificity
- From:Journal of Laboratory Medicine and Quality Assurance
2007;29(2):247-252
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Cefoxitin, a cephamycin-type antibiotic, is known to be superior to oxacillin in predicting the presence of mecA gene because it serves as a very potent inducer of mecA regulatory system. We used a cefoxitin disk diffusion methods for detection of methicillin-resistant Staphylococcus aureus (MRSA), and compared it with the conventional methods. METHODS: For 50 MRSA and 50 methicillin susceptible S. aureus confirmed by mecA and femA gene PCR, oxacillin, cefoxitin, and moxalactam disk diffusion methods, oxacillin and cefoxitin E-tests, Vitek 2 and Microscan Walkaway antibiotics susceptibility tests, and PBP2a latex agglutination test were performed. The sensitivity and specificity of each method were evaluated. RESULTS: The sensitivities of oxacillin disk diffusion method and E-test were 96%. The sensitivities of cefoxitin and moxalactam disk diffusion method, cefoxitin E-test, Vitek 2, Microscan Walkaway, PBP2a latex agglutination test were 100%. The specificities were 100% for all the methods used. CONCLUSIONS: It may be considered that both the cefoxitin- and moxalactam disk diffusion methods are effective and excellent screening methods for the detection of MRSA in clinical laboratory routinely.
