Comparison of Radiation Adaptive Responses in Peripheral Lymphocytes of Patients Undergoing Tc-99m MDP and Tc-99m DTPA Scintigraphies.
- Author:
Hee Seung ROM
;
Ming Hao LI
;
Jung Jun MIN
;
An Sung KWON
;
Ji Yeul KIM
- Publication Type:Original Article
- Keywords:
Radiation adaptive response;
Peripheral lymphocyte;
Chromosomal aberration;
Tc-99m methylene diphosphonate (MDP);
Tc-99m diethylenetriamine pentaaeetric acid (DTPA)
- MeSH:
Chromosome Aberrations;
Female;
Humans;
Lymphocytes*;
Male;
Pentetic Acid*;
Technetium Tc 99m Medronate*
- From:Korean Journal of Nuclear Medicine
2000;34(3):252-259
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study was to compare the radiation adaptive response (RAR) in peripheral lymphocytes (PL) of patients induced by Tc.-99m MDP and Tc-99m DTPA scintigraphies. MATERIALS AND METHODS: Lymphocytes from 45 patients (25 males, 20 females, mean age 44+/-18 years) were collected before and after scintigraphies using 740 MBq Tc-99m MDP (n=22) or Tc-99m L)TPA (n=23). Lympho-cytes from 20 controls (12 males, 8 females, mean age 43+/-7 years) were also callected. They were exposed to challenge dose of 2 Gy gamma-rays using a Cs-137 cell irradiator, Number of ring-form (R) and dicentric (D) chromosomes was counted under the light microscope. From them a representative score, Ydr, was calculated as Ydr=(D+R)/cells. Adaptation index (AI) was defined as difference of Ydr between unconditioned and conditioned lymphocytes. Ydr was also measured after an administration of cyclohexi-mide (CHM), a protein synthesis inhibitor, before challenge dose. RESULTS: RAR was induced in both groups of patients. CHM abolished the adaptive response in both groups. AI of Tc-99m MDP group was significantly higher than that of Tc-99m DTPA group. CONCLUSION: Tc-99m MDP induced RAR was more prominent than those induced by Tc-99m DTPA.
