Clathrin Assembly Lymphoid Myeloid Leukemia-AF10-positive Acute Leukemias: A Report of 2 Cases with a Review of the Literature.
10.3343/kjlm.2010.30.2.117
- Author:
Ji Young HUH
1
;
Soie CHUNG
;
Doyeun OH
;
Myung Seo KANG
;
Hyeon Seok EOM
;
Eun Hae CHO
;
Mi Hwa HAN
;
Sun Young KONG
Author Information
1. Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea.
- Publication Type:Case Report ; Review
- Keywords:
t(10;
11);
CALM-AF10;
HOXA gene;
hDOT1L;
Acute leukemia;
Reverse transcriptase-PCR
- MeSH:
Adolescent;
Adult;
Bone Marrow/pathology;
Chromosomes, Human, Pair 10;
Chromosomes, Human, Pair 11;
Cord Blood Stem Cell Transplantation;
Female;
Histone-Lysine N-Methyltransferase/genetics/metabolism;
Homeodomain Proteins/genetics/metabolism;
Humans;
Leukemia, Myeloid, Acute/diagnosis/*genetics/therapy;
Male;
Monomeric Clathrin Assembly Proteins/*genetics;
Oncogene Proteins, Fusion/*genetics;
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/*genetics/therapy;
Recurrence;
Transcription Factors/*genetics;
Translocation, Genetic
- From:The Korean Journal of Laboratory Medicine
2010;30(2):117-121
- CountryRepublic of Korea
- Language:English
-
Abstract:
The translocation t(10;11)(p13;q14q21) has been found to be recurrent in acute lymphoblastic and myeloid leukemias, and results in the fusion of the clathrin assembly lymphoid myeloid leukemia (CALM) gene with the AF10 gene; these genes are present on chromosomes 11 and 10, respectively. Because the CALM-AF10 rearrangement is a rare chromosomal abnormality, it is not included in routine molecular tests for acute leukemia. Here, we describe the cases of 2 patients with the CALM-AF10 fusion gene. The first patient (case 1) was diagnosed with T-cell ALL, and the second patient (case 2) was diagnosed with AML. Both patient samples showed expression of the homeobox A gene cluster and the histone methyltransferase hDOT1L, which suggests that they mediate leukemic transformation in CALM-AF10-positive and mixed-lineage leukemia-AF10-positive leukemias. Both patients achieved complete remission after induction chemotherapy. The first patient (case 1) relapsed after double-unit cord blood transplantation; there was no evidence of relapse in the second patient (case 2) after allogenic peripheral blood stem cell transplantation. Since CALM-AF10- positive leukemias have been shown to have poor prognosis with conventional therapy, molecular tests for CALM-AF10 rearrangement would be necessary to detect minimal residual disease during follow-up.
