Xpert CARBA-R Assay for the Detection of Carbapenemase-Producing Organisms in Intensive Care Unit Patients of a Korean Tertiary Care Hospital.
10.3343/alm.2016.36.2.162
- Author:
Do Kyun KIM
1
;
Hyoung Sun KIM
;
Naina PINTO
;
Jongsoo JEON
;
Roshan D'SOUZA
;
Myung Sook KIM
;
Jun Yong CHOI
;
Dongeun YONG
;
Seok Hoon JEONG
;
Kyungwon LEE
Author Information
1. Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea. deyong@yuhs.ac
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Carbapenemase-producing organisms;
Carbapenem-resistant organisms;
Intensive care unit;
Colonization;
Xpert CARBA-R
- MeSH:
Anti-Bacterial Agents/pharmacology;
Bacterial Proteins/*genetics/metabolism;
DNA, Bacterial/analysis;
Drug Resistance, Multiple, Bacterial/genetics;
Enterobacteriaceae/drug effects/genetics/*isolation & purification;
Feces/microbiology;
Humans;
Imipenem/pharmacology;
Intensive Care Units;
Klebsiella pneumoniae/drug effects/genetics/*isolation & purification;
Reagent Kits, Diagnostic;
Real-Time Polymerase Chain Reaction;
Republic of Korea;
Tertiary Healthcare;
beta-Lactamases/*genetics/metabolism
- From:Annals of Laboratory Medicine
2016;36(2):162-165
- CountryRepublic of Korea
- Language:English
-
Abstract:
Carbapenemase-producing organisms (CPO) are rapidly disseminating worldwide, and their presence in tertiary care hospitals poses a significant threat to the management of nosocomial infections. There is a need to control CPO, especially in intensive care unit (ICU) patients, because these organisms are resistant to most beta-lactam antibiotics and are easily transmitted. At present, the identification of CPO is time-consuming; hence, this study focused on the use of the Xpert CARBA-R assay (Cepheid, USA) to determine intestinal colonization rates of CPO in patients admitted to the ICU of a tertiary care hospital in Korea. Forty clinical stool samples were collected and inoculated both in a CARBA-R cartridge and in conventional culture plates. The CARBA-R assay required only ~one hour to screen CPO, while the time required for conventional culture was over three days. We also found that the prevalences of intestinal colonization by carbapenem-resistant organisms and Enterobacteriaceae were 17.5% (7 out of 40) and 7.5% (3 out of 40), respectively. Among the colonizing strains, three that contained carbapenemase, including Klebsiella pneumonia carbapenemase (KPC), and imipenem (IMP) and Verona integron-mediated metallo-beta-lactamase (VIM) were found. With its convenience, the Xpert CARBA-R assay can be included in CPO surveillance strategies.
