- Author:
Tae Sang OH
1
;
Hee Yoon KANG
;
You Sun NAM
;
Young Jin KIM
;
Eun Kyung YOU
;
Min Young LEE
;
Sun Young CHO
;
Hee Joo LEE
Author Information
- Publication Type:Brief Communication
- Keywords: Mycobacterium tuberculosis; RNA extraction; Quantitative reverse transcription polymerase chain reaction
- MeSH: Cell Wall; Mycobacterium tuberculosis*; Mycobacterium*; Mycolic Acids; RNA*
- From:Annals of Clinical Microbiology 2016;19(1):20-23
- CountryRepublic of Korea
- Language:English
- Abstract: In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid, which renders the cells resistant to lysis. Using TRIzol reagent and several powerful bead-beating steps, a high quantity of RNA was obtained.