An Effective Method of RNA Extraction from Mycobacterium tuberculosis.

Author: Tae Sang OH ¹ ; Hee Yoon KANG ; You Sun NAM ; Young Jin KIM ; Eun Kyung YOU ; Min Young LEE ; Sun Young CHO ; Hee Joo LEE
Author Information

1. Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Korea.
Publication Type:Brief Communication
Keywords: Mycobacterium tuberculosis; RNA extraction; Quantitative reverse transcription polymerase chain reaction
MeSH: Cell Wall; Mycobacterium tuberculosis*; Mycobacterium*; Mycolic Acids; RNA*
From:Annals of Clinical Microbiology 2016;19(1):20-23
CountryRepublic of Korea
Language:English
Abstract: In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid, which renders the cells resistant to lysis. Using TRIzol reagent and several powerful bead-beating steps, a high quantity of RNA was obtained.