A Study of Cytotoxical Mechanism of Mercurial Compounds in RAW264.7 Cell Line.
- Author:
Kong Ho KIRN
1
;
Byoung Yul SOH
;
Dai Ha KOH
Author Information
1. Department of Preventive Medicine and Public Health, School of Medicine and Institute for Medical Science Chonbuk National University, Korea.
- Publication Type:Original Article
- Keywords:
Nitrite;
Nitric Oxide;
EMT-6 Cell;
Mercury Chloride;
Methylmercury chloride;
glucose
- MeSH:
Adenosine Triphosphate;
Cell Culture Techniques;
Cell Line*;
Citric Acid Cycle;
Cytokines;
Glucose;
Hydrogen-Ion Concentration;
Immunity, Cellular;
Interleukin-1;
Mercury Compounds;
Nitric Oxide
- From:Korean Journal of Occupational and Environmental Medicine
1996;8(3):560-569
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The effects of glucose on the productions of ATP and nitrite which are inhibited by mercury compounds, were examined in a cell culture system of RAW 264.7 cells. The cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) with cytokines, IL-1 and TNF for 24 hours. The viablility of RAW 264.7 cells at the end of culture was significantly decreased by mercury chloride or methylmercury chloride added into the media in dose-dependent manner, however the viability of RAW 264.7 cells were influenced in the concentrations lese than 0.8micrometer of mercury chloride or 0.4micrometer of methylmercury chloride. The addition of 4.5 g/l glucose to normal DMEM lowered the pH of media to the range of 6.7-6.8 after 48 hours of culture, but not for the cell survivals. This supplement of glucose to the media also prevented the inhibitions of ATP and nitrite syntheses which were caused by mercurial compounds. These results suggest that the disorder of cell mediated immunity by mercurials could be related to the inhibition of nitric oxide synthesis which seams to be caused by the inhibition of ATP synthesis, especially related to the citric acid cycle.
