Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine.
10.4142/jvs.2009.10.3.177
- Author:
Juyeon LEE
1
;
Changjong MOON
;
Jihoon KIM
;
Chanwoo JUNG
;
Keun Hwa LEE
;
Hong Gu JOO
;
Meejung AHN
;
Taekyun SHIN
Author Information
1. Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, Korea. shint@cheju.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
bovine paratuberculosis;
galectin-3;
granulation;
intestine
- MeSH:
Animals;
Cattle;
Cattle Diseases/*pathology;
Chronic Disease;
Galectin 3/*metabolism;
Immunohistochemistry;
Intestine, Small/microbiology/*pathology;
Mycobacterium avium subsp. paratuberculosis/growth & development/isolation & purification;
Paratuberculosis/*pathology;
RNA Polymerase II/genetics
- From:Journal of Veterinary Science
2009;10(3):177-180
- CountryRepublic of Korea
- Language:English
-
Abstract:
The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl-Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum. These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.
