Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice.
10.4142/jvs.2009.10.3.189
- Author:
Jin Joo HUE
1
;
Ki Nam LEE
;
Jae Hwang JEONG
;
Sang Hwa LEE
;
Young Ho LEE
;
Seong Woon JEONG
;
Sang Yoon NAM
;
Young Won YUN
;
Beom Jun LEE
Author Information
1. College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea. beomjun@cbu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
conjugated linoleic acid;
C57BL/6J ob/ob mouse;
diglyceride;
obesity;
PPAR-gamma
- MeSH:
Adipose Tissue/*drug effects;
Animals;
Anti-Obesity Agents/*pharmacology;
Blood Chemical Analysis;
Body Weight/*drug effects;
Diglycerides/*pharmacology;
Disease Models, Animal;
Eating/drug effects;
Gene Expression Regulation/drug effects;
Linoleic Acids, Conjugated/*pharmacology;
Lipids/blood;
Liver/drug effects/pathology;
Male;
Mice;
Mice, Inbred C57BL;
Mice, Obese;
Obesity/*metabolism/pathology;
PPAR gamma/metabolism;
Time Factors
- From:Journal of Veterinary Science
2009;10(3):189-195
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p < 0.01). All test solutions significantly decreased the levels of triglyceride, glucose and free fatty acids in the serum compared with control (p < 0.05). The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05). CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05). DG and DG-CLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05). A remarkable decrease in the number of lipid droplets and fat globules was observed in the livers of mice treated with DG, CLA, and DG-CLA compared to control. Treatments of DG and CLA actually increased the expression of peroxisome proliferator-activated receptor gamma. These results suggest that DG-CLA containing 22% CLA have a respectable anti-obesity effect by controlling serum lipids and fat metabolism.
