Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells.
10.4142/jvs.2009.10.3.197
- Author:
Mohamed Mohamed SOLIMAN
1
;
Yakut Abdel-Fattah EL-SENOSI
;
Maysara Mahmoud SALEM
;
Omniya Mahmoud Abdel HAMID
;
Kimura KAZUHIRO
Author Information
1. Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, 020-013, Egypt. mohamedsoliman8896@yahoo.com
- Publication Type:Original Article ; In Vitro
- Keywords:
acylation stimulating protein;
adipogenesis;
3T3L-1 cells
- MeSH:
3T3 Cells;
Adipogenesis/*drug effects;
Animals;
Gene Expression Regulation/*drug effects;
Hypoglycemic Agents/pharmacology;
Insulin/pharmacology;
Intercellular Signaling Peptides and Proteins/*pharmacology;
Lipid Metabolism/drug effects;
Mice;
Protease Inhibitors/*pharmacology;
Time Factors
- From:Journal of Veterinary Science
2009;10(3):197-201
- CountryRepublic of Korea
- Language:English
-
Abstract:
Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p < 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 microgram/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.
