Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand.
10.4142/jvs.2009.10.3.219
- Author:
Tawatchai POHUANG
1
;
Niwat CHANSIRIPORNCHAI
;
Achara TAWATSIN
;
Jiroj SASIPREEYAJAN
Author Information
1. Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. cniwat@chula.ac.th
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
infectious bronchitis virus;
phylogenetic analysis;
S1 gene;
sequencing;
Thailand
- MeSH:
Animals;
Chickens;
Coronavirus Infections/*veterinary/virology;
Infectious bronchitis virus/*genetics/*isolation & purification;
Molecular Sequence Data;
Phylogeny;
Poultry Diseases/*virology;
Sequence Homology, Amino Acid;
Sequence Homology, Nucleic Acid;
Thailand;
Viral Proteins/chemistry
- From:Journal of Veterinary Science
2009;10(3):219-223
- CountryRepublic of Korea
- Language:English
-
Abstract:
Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and amino acid sequence identities less than 84% in common with IBVs published in the GenBank database. This group likely represents the strains indigenous to Thailand. The isolates in group II showed a close relationship with Chinese IBVs. They had nucleotide sequence identities of 97-98% and amino acid sequence identities 96-98% in common with Chinese IBVs (strain A2, SH and QXIBV). This finding indicated that the recent Thai IBVs evolved separately and at least two groups of viruses are circulating in Thailand.
