Role of Integrin, FAK (Focal Adhesion Kinase) and ERK (Extracellular Signal Regulated Kinase) on the Suppressed Cell Proliferation of Endometrial Cancer Cells by GnRH (Gonadotropin-Releasing Hormone).
- Author:
Jong Rak CHOI
;
Dong Wook PARK
;
Dong Soon CHOI
;
Churl K MIN
- Publication Type:Original Article
- Keywords:
GnRH;
Integrin;
FAK;
ERK;
Phosphorylation
- MeSH:
Cell Proliferation*;
Endometrial Neoplasms*;
Female;
Gonadotropin-Releasing Hormone*;
Humans;
Immunoblotting;
Integrin beta3;
Phosphorylation;
Signal Transduction;
Thymidine
- From:Korean Journal of Fertility and Sterility
2006;33(2):115-123
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II. METHOD: Human endometrial cancer cells (HEC1A) were cultured under the following condition: DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [3H] thymidine incorporation assay. Immunoblotting was utilized to detect proteins. RESULTS: GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin beta3. Phosphorylation of FAK and ERK were induced by GnRH-I and -II. CONCLUSION: GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation.
