Change of alpha-SM Actin Expression Induced by the Antibody for TGF-beta in Fibroblast NIH3T3 Culture: The basic research for the inhibition of wound contracture.
- Author:
Bong Hwa LEE
1
;
Hyung Kil KANG
;
Jeong Jin KIM
;
Dong Kun KIM
;
Dae Won YOON
Author Information
1. Department of Surgery, Hallym University College of Medicine, Seoul, Korea. bshlee@hallym.or.kr
- Publication Type:Original Article
- Keywords:
Wound healing;
Wound contracture;
Myofibroblast;
TGF-beta;
Smooth muscle actin
- MeSH:
Actins*;
Antibodies;
Autoradiography;
Blotting, Western;
Cell Culture Techniques;
Contracture*;
Electrophoresis, Polyacrylamide Gel;
Fibroblasts*;
Myofibroblasts;
Transforming Growth Factor beta*;
Wound Healing;
Wounds and Injuries*
- From:Journal of the Korean Society of Coloproctology
2001;17(3):113-118
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this experiment is to measure the expression of TGF-beta and alpha-SM actin (smooth muscle actin) from fibroblast culture by the duration of culture days and to analyze the inhibition of alpha-SM actin expression in fibroblast by the antibodies for TGF-beta. METHODS: The levels of alpha-SM actin from the paired NIH3T3 cell cultures with TGF-beta 1 containing medium (10 ng/ml) and with the antibody (for TGF-beta) medium (1 or 2 ug/ml) were determined by SDS PAGE for cell lysate protein, Western blot with ECL autoradiography and immuno - slot blot. RESULTS: In NIH3T3 culture, the expression of alpha-SM actin increased at culture days 4, 5, 6. TGF-beta was expressed from 2nd day of culture and increased by day 7. The addition of TGF beta (10 ng/ml) did not increased the expression of alpha-SM actin. But alpha-SM actin expression decreased in the presence of anti-TGF beta antibody. The decrease of expression was proportional to the concentration of antibody and duration of exposure to the antibody. CONCLUSIONS: Endogenous TGF-beta produced by fibroblast cultures is sufficient to express the alpha-SM actin from the myofiboblast. There was no additive expression of alpha-SM actin with exogenous TGF-beta 1. The antibody for TGF- beta inhibits the production of the alpha-SM actin during wound healing and may prevent the wound contracture.
