Caveolin-1 is involved in high glucose accelerated human glomerular mesangial cell senescence.
- Author:
Xin FENG
1
;
Wei GAO
;
Yao LI
Author Information
- Publication Type:Original Article
- Keywords: Mesangial cells; Aging; Caveolin-1
- MeSH: Aging*; Blotting, Southern; Blotting, Western; Caveolin 1*; Cell Aging; Glucose*; Humans*; Mesangial Cells*; Telomere
- From:The Korean Journal of Internal Medicine 2017;32(5):883-889
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND/AIMS:: We demonstrated the role of caveolin-1 involved in high glucose (HG)-induced glomerular mesangial cells (GMCs) senescence. METHODS:: HG was used to stimulate GMCs. The telomere lengths were analyzed by Southern blot. β-Galactosidase staining was determined. The expressions of caveolin-1 and P53 proteins were determined by Western blot. RESULTS:: Treatment with high concentrations of glucose induced GMC senescence accompanied by shortened telomere length and increase of β-galactosidase staining as well as P53 protein, which was abrogated after application of caveolin-1-siRNA. CONCLUSIONS:: This study proved that HG induced cell senescence in GMCs. The caveolin-1 is involved in HG-induced mesangial cell senescence, and blocking caveolin-1 significantly reduced cell senescence. The effect of caveolin-1 is mediated by P53 pathway.
