Genotype of Toxoplasma gondii from Blood of Stray Cats in Gyeonggi-do, Korea.
10.3347/kjp.2009.47.4.413
- Author:
Hye Youn KIM
1
;
Yun Ah KIM
;
Ho Sa LEE
;
Ho Gun RHIE
;
Shin Hyeong CHO
;
Jae Ran YU
;
Sang Eun LEE
Author Information
1. Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul 122-701, Korea. ondalgl@cdc.go.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii;
PCR-RFLP;
stray cat;
SAG2
- MeSH:
Animals;
Antigens, Protozoan/genetics;
Blood/*parasitology;
Cat Diseases/*parasitology;
Cats;
Cluster Analysis;
DNA Fingerprinting/methods;
DNA, Protozoan/genetics/isolation & purification;
Genotype;
Korea;
Polymerase Chain Reaction/methods;
Polymorphism, Restriction Fragment Length;
Protozoan Proteins/genetics;
Toxoplasma/*classification/*genetics/isolation & purification;
Toxoplasmosis, Animal/*parasitology
- From:The Korean Journal of Parasitology
2009;47(4):413-415
- CountryRepublic of Korea
- Language:English
-
Abstract:
Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2 gene, amplifying the 5'and 3'ends of the locus. The results revealed that all samples belonged to the type I clonal lineage. Although T. gondii organisms were not isolated from the samples, the results of the present study represent that stray cats with T. gondii infection should be seriously concerned in our environment. Adequate and continuous control programs of stray cats are needed to reduce the risk of transmission of T. gondii as a zoonotic infection threatening the public health.
