NMDA Receptor Activation Mediates Neuropathic Pain States Induced by Calcium Channel alpha2delta1 Subunit.
10.3344/kjp.2009.22.3.210
- Author:
Soo Bong YU
1
;
Young Soo LIM
;
Doo Sik KIM
Author Information
1. Department of Anesthesiology and Pain Medicine, Kosin University College of Medicine, Busan, Korea. kdsshj@ns.kosinmed.or.kr
- Publication Type:Original Article
- Keywords:
allodynia;
calcium channel alpha2delta1 subunit;
memantine;
NMDA receptor
- MeSH:
Animals;
Calcium;
Calcium Channels;
Hyperalgesia;
Hypersensitivity;
Injections, Spinal;
Memantine;
Mice;
Mice, Transgenic;
N-Methylaspartate;
Negotiating;
Neuralgia;
Rats;
Rats, Sprague-Dawley;
Receptors, N-Methyl-D-Aspartate;
Sensory Receptor Cells;
Spinal Cord;
Spinal Nerves;
Up-Regulation
- From:The Korean Journal of Pain
2009;22(3):210-215
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Several studies have indicated that a nerve injury enhances the expression of the voltage-gated calcium channel alpha2delta1 subunit (Cavalpha2delta1) in sensory neurons and the dorsal spinal cord. This study examined whether NMDA receptor activation is essential for Cavalpha2delta1-mediated tactile allodynia in Cavalpha2delta1 overexpressing transgenic mice and L5/6 spinal nerve ligated rats (SNL). These two models show similar Cavalpha2delta1 upregulation and behavioral hypersensitivity, without and with the presence of other injury factors, respectively. METHODS: The transgenic (TG) mice were generated as described elsewhere (Feng et al., 2000). The left L5/6 spinal nerves in the Harlan Sprague Dawley rats were ligated tightly (SNL) to induce neuropathic pain, as described by Kim et al. (1992). Memantine 2 mg/kg (10 ul) was injected directly into the L5/6 spinal region followed by 10microl saline. Tactile allodynia was tested for any mechanical hypersensitivity. RESULTS: The tactile allodynia in the SNL rats could be reversed by an intrathecal injection of memantine 2 mg/kg at 1.5 hours. The tactile allodynia in the Cavalpha2delta1 over-expressing TG mice could be reversed by an intrathecal injection of memantine 2 mg/kg at 1.5, 2.0 and 2.5 hours. CONCLUSIONS: The behavioral hypersensitivity was similar in the TG mice and nerve injury pain model, supporting the hypothesis that elevated Cavalpha2delta1 mediates similar pathways that underlie the pain states in both models. The selective activation of spinal NMDA receptors plays a key role in mediating the pain states in both the nerve-injury rats and TG mice.
