Significance of low cytometric DNA histogram in bladder cancer.
- Author:
Nam Cheol PARK
1
;
Jong Byung YOON
Author Information
1. Department of Urology, College of Medicine, Pusan National University, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
Bladder cancer;
Flow cytometry;
DNA ploidy
- MeSH:
Busan;
Carcinoma in Situ;
Carcinoma, Transitional Cell;
Comprehension;
Cystectomy;
DNA*;
Epithelium;
Flow Cytometry;
Mucous Membrane;
Natural History;
Ploidies;
Population Characteristics;
Recurrence;
Survival Rate;
Urinary Bladder Neoplasms*;
Urinary Bladder*
- From:Korean Journal of Urology
1991;32(2):215-227
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To investigate tumor heterogeneity and the relationship between DNA ploidy and histopathological parameters. flow cytometric assessments were performed on fifteen control bladders and 77 transitional cell carcinomas of bladder confirmed histopathologically. Between August 1984 and July 1990 in Pusan National University Hospital. 31 TUR surgeries and 46 total cystectomies were done and are included in this series. The cystectomized specimens were topographically evaluated with bladder mapping method ; 146 multiple formalin-fixed paraffin-embedded blocks (1-6 blocks with s mean of 3.0) were selected from the histologically different sites including main exophytic mass. concomitant carcinoma in situ. and neighboring normal bladder epithelium. Thirty one TUR blocks (one per case) with histopathological reviews were selected. The results were as follows 1. Flow cytometry positive rates revealed 58.1% in the TUR group and 76.1% in the cystecbmy group 2. Flow cytometry positive rates according to histopathological stage revealed 43.8% and 73.3% of Ta and T1. respectively, in the TUR group, so that in T1 the positive rate is nearly twice that of Ta. Flow cytometry positive rates revealed 100.0%, 70.0%. 100.0%, 66.6% and 77.8% of Tis. T1. T2. T3 and T4, respectively. in the cystectomy group, so that no significant differences were observed between each T-category.3. Flow cytometry positive rates according to histopathological grade revealed 38.5%, 70.6% and 100.0% or grade I, II and III in the TUR group. and O%, 68.0% and 90.0% in the cystectomy group. respectively. So positive rates proportionally increased with up-grading in both TUR end cystectomy groups. 4. Intratumoral ploidy heterogeneity of main exophytic mass in the cystectomy group was in 58.3%, and intertumoral ploidy heterogeneity between main exophytic mass and concomitant carcinoma in situ was 51.7%. 5. DNA ploidy assessment of nontumorous mucosa was thought to provide valuable information for the comprehension of natural history in bladder cancer. 6. DNA ploidy had a significant relationship with the recurrence rates in the TUR group, but was not correlated with the survival rates in contrast to grade and stage in the cystectomy group.According to the above results, we thought that flow cytometric DNA assessment was valuable in evaluating the multicentricity in origin with heterogeneity and to predict the malignant potential as an adjunct of the histopathological factors in bladder cancer.
