Identification and purification of IgE-reactive proteins in German cockroach extract.
10.3349/ymj.1999.40.3.283
- Author:
Tai Soon YONG
1
;
Jong Seok LEE
;
Jongweon LEE
;
Soon Jung PARK
;
Soung Hoo JEON
;
Han Il REE
;
Jung Won PARK
Author Information
1. Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea. tsyong212@yumc.yonsei.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
German cockroach;
IgE;
allergens;
immunoblot;
amino acid sequence
- MeSH:
Amino Acid Sequence/genetics;
Animal;
Cockroaches/chemistry*;
Human;
IgE/immunology*;
Insect Proteins/isolation & purification*;
Insect Proteins/immunology*;
Insect Proteins/genetics;
Mice;
Mice, Inbred BALB C;
Tissue Extracts/chemistry*
- From:Yonsei Medical Journal
1999;40(3):283-289
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cockroaches have been implicated as a cause of respiratory allergy in urban areas worldwide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50, 43 and 36 KD by immunoblot analysis in both immune BALB/c mice and sensitized humans. Prominent IgE-reactive proteins were purified using FPLC by ion-exchange chromatography, gel filtration and hydrophobic chromatography. The N-terminal amino acid sequence of a purified protein with a MW of 66 KD on SDS-PAGE was Val-Thr-Leu-Lys-Lys(Val)-Met-Ile-Lys-Thr-Phe-Tyr. No homologous protein was found through a search of GenBank that indicated a novel IgE-reactive protein in German cockroach extract. Another purified protein with a MW of 36 KD reacted strongly with a monoclonal antibody against Bla g 2.
