Effects of Interleukin-2 Transduction into the Human Hepatoma Cell Lines Using Retroviral Vector.
- Author:
Soo Jung GONG
1
;
Nae Chun YOO
;
Joo Hang KIM
;
Dong Hwan SHIN
;
Hyo Dong UHM
;
Sook Jung JEONG
;
Jae Yong CHO
;
Sun Young RHA
;
Yeon Soo KIM
;
Hyun Cheol CHUNG
;
Jae Kyung ROH
;
Jin Sik MIN
;
Byung Soo KIM
Author Information
1. Department of Internal Medicine, Yonsei University, College of Medicine, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Interleukin-2;
Hepatoma cell line;
Tumor regression
- MeSH:
Animals;
Carcinoma, Hepatocellular*;
Cell Line*;
Cell Line, Tumor;
Enzyme-Linked Immunosorbent Assay;
Humans*;
Interleukin-2*;
Mice;
Mice, Nude;
Monocytes;
Parents;
Retroviridae;
Zidovudine*
- From:Journal of the Korean Cancer Association
1997;29(4):555-564
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We compared the differences between parent hepatoma cell lines and interleukin-2 (IL-2) transduced hepatoma cell lines using N2A/IL-2 and LNC/IL-2 retrovirus with regards to in vitro sensitivity to peripheral blood monocytes and in vivo tumorigenic activity. MATERIALS AND METHODS: Retroviral vector and producer cell line were constructed and IL-2 gene was transduced into the human hepatoma cell lines (SK-Hep1, Hep-G2, Hep-3B). IL-2 secretion after IL-2 transduction was measured by ELISA. MTT assay for in vitro sensitivity to peripheral blood monocytes was performed and the tumorigenic activity was observed in BALB/c mice and nude mice. RESULTS: IL-2 secretion was 186 pg/10 degrees C cells/24 hrs in SK-Hep1 cell line and was 147 pg/10 (6) cells/24 hrs in Hep-3B cell line with N2A/IL-2 retroviral vector and was 55,000 pg/10 (6) cells/24 hrs with LNC/IL-2 retroviral vector. In vitro sensitivity to peripheral blood monocytes was increased by 163.8~254% in IL-2 transduced hepatoma cell lines (Hep -3B/N2A/IL-2, Hep-G2/N2A/IL-2) compared to those of the parent cell lines. The tumorigenicity was observed in 1 of 3 BALB/c mice and all 3 nude mice. Simultaneous injection of 1 X 10 (7) cells of the parent cell line (Hep-3B) into the right flank and IL-2 transduced cell line (Hep-3B/LNC/IL-2) into the left flank of the three BALB/c mice and of 5 X 10 (5) cells for the three nude mice resulted in a complete regression of the IL-2 modified tumor cell line (Hep-3B/LNC/IL-2) in 3 weeks and the parent cell line (Hep-3B) in 5 weeks. But, after the injection of 1.5 X 10 (7) cells for other five nude mice, the tumor of the IL-2 transduced hepatoma cell line (Hep-3B/LNC/IL-2) was gradually disappeared, and the tumor of the parent hepatoma cell line (Hep-3B) was initially decreased and then gradually regrew 20 days later. CONCLUSION: IL-2 transduced hepatoma cell lines secreting IL-2 became more sensitive to peripheral blood monocytes and resulted in the increased antigenicity to the tumors formed by IL-2 transduced hepatoma cell line and parent cell line, and finally resulted in the regression of the tumors in experimental animals.
