Prevalence of Anaplasma, Bartonella and Borrelia Species in Haemaphysalis longicornis collected from goats in North Korea.
10.4142/jvs.2016.17.2.207
- Author:
Jun Gu KANG
1
;
Sungjin KO
;
W Barney SMITH
;
Heung Chul KIM
;
In Yong LEE
;
Joon Seok CHAE
Author Information
1. Laboratory of Veterinary Internal Medicine, BK21 PLUS Program for Creative Veterinary Science Research, and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea. jschae@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Anaplasma;
Bartonella;
Borrelia;
Haemaphysalis longicornis;
North Korea
- MeSH:
Anaplasma phagocytophilum;
Anaplasma*;
Animals, Domestic;
Bartonella henselae;
Bartonella*;
Borrelia*;
China;
Databases, Nucleic Acid;
Democratic People's Republic of Korea*;
Far East;
Goats*;
Humans;
Korea;
Male;
Nymph;
Polymerase Chain Reaction;
Prevalence*;
RNA, Ribosomal, 16S;
Ticks
- From:Journal of Veterinary Science
2016;17(2):207-216
- CountryRepublic of Korea
- Language:English
-
Abstract:
North Korea is located on the northern part of the Korean Peninsula in East Asia. While tick-borne pathogens of medical and veterinary importance have been reported from China and South Korea, they have not been reported from North Korea. To screen for zoonotic tick-borne pathogens in North Korea, ticks were collected from domestic goats. A total of 292 (27 nymph, 26 male, 239 female) Haemaphysalis (H.) longicornis were collected and assayed individually for selected tick-borne pathogens. A total of 77 (26.4%) were positive for Anaplasma bovis, followed by Bartonella (B.) grahamii (15, 5.1%), Anaplasma phagocytophilum (12, 4.1%), Bartonella henselae (10, 3.4%), and Borrelia spp. (3, 1.0%) based on 16S ribosomal RNA and ITS species-specific nested polymerase chain reaction. Using the groEL-based nested PCR, a total of 6 and 1 H. longicornis were positive for B. grahamii and B. henselae, respectively. All products were sequenced and demonstrated 100% identity and homology with previously reported sequences from other countries in GenBank. This is the first report of the detection of tick-borne pathogens in the North Korea and suggests that farm animals may act as reservoirs for zoonotic tick-borne pathogens.
