Intactness of zona pellucida does not affect the secretion of a trypsin-like protease from mouse blastocyst.
10.3346/jkms.2000.15.5.529
- Author:
Seong Soo HWANG
1
;
Eun Young LEE
;
Yung Chai CHUNG
;
Byung Koo YOON
;
Je Ho LEE
;
Doo Seok CHOI
Author Information
1. IVF Clinic, Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Protease Activity;
Zona Pellucida;
Mouse Embryo
- MeSH:
Animal;
Blastocyst/secretion;
Blastocyst/enzymology*;
Female;
Fertilization in Vitro/methods;
Gonadotropins, Chorionic/pharmacology;
Mice;
Mice, Inbred C57BL;
Mice, Inbred CBA;
Pregnancy;
Serine Endopeptidases/secretion;
Serine Endopeptidases/metabolism*;
Zona Pellucida/physiology*;
Zona Pellucida/drug effects
- From:Journal of Korean Medical Science
2000;15(5):529-532
- CountryRepublic of Korea
- Language:English
-
Abstract:
Assisted hatching (AH), which is known to improve the hatching potential of mammalian embryos, has been used to increase the pregnancy rate in in vitro fertilization cycles. However, the effect of AH on a trypsin-like protease, which is known to be associated with the hatching process, has not been studied. In this study, we evaluate whether the intactness of zona pellucida affects the secretion of a trypsin-like protease from mouse blastocyst. Four- to 8-cell stage mouse embryos were collected at 66- to 68 hr after hCG injection and divided into 3 groups according to the manipulation of zona pellucida. The groups are no treatment (control), drilling of zona pellucida (ZD) and thinning of zona pellucida (ZT). The activity of a trypsin-like protease, blastocyst development and hatching rate were compared among the three groups at 110 and 135 hr after hCG injection, respectively. The protease activity and blastocyst development were not significantly different among control, ZD and ZT groups at 110 and 135 hr after hCG injection, respectively. However, the hatching rate of ZD and ZT groups was significantly higher than that of control group at each time, respectively (p>0.001). Even in the zona pellucida removed embryos, the protease activity did not differ from the control group. In conclusion, the secretion of a trypsin-like protease from mouse blastocyst does not seem to be affected by the intactness of zona pellucida.
