Effects of Antioxidant on Reduction of Hindlimb Muscle Atrophy Induced by Cisplatin in Rats.
10.4040/jkan.2014.44.4.371
- Author:
Jin Il KIM
1
;
Myoung Ae CHOE
Author Information
1. Department of Nursing, Christian College of Nursing, Gwangju, Korea. neoreva@hanmail.net
- Publication Type:Original Article ; English Abstract
- Keywords:
Antioxidant;
Cisplatin;
Rat;
Muscular atrophy;
Superoxide dismutase
- MeSH:
Animals;
Body Weight/drug effects;
Cisplatin/*toxicity;
Disease Models, Animal;
Extracellular Signal-Regulated MAP Kinases/metabolism;
Hindlimb;
Male;
Muscle, Skeletal/*drug effects/enzymology/metabolism;
Muscular Atrophy/*chemically induced/metabolism/pathology;
Phosphorylation;
Proto-Oncogene Proteins c-akt/metabolism;
Rats;
Rats, Sprague-Dawley;
Recombinant Proteins/biosynthesis/genetics/pharmacology;
Superoxide Dismutase/genetics/metabolism/pharmacology;
Superoxides/metabolism
- From:Journal of Korean Academy of Nursing
2014;44(4):371-380
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study was to examine the effects of Cu/Zn SOD on reduction of hindlimb muscular atrophy induced by cisplatin in rats. METHODS: Forty-two rats were assigned to three groups; control group, Cisplatin (CDDP) group and cisplatin with Cu/Zn SOD (CDDP-SOD) group. At day 35 hindlimb muscles were dissected. Food intake, activity, withdrawal threshold, muscle weight, and Type I, II fiber cross-sectional area (CSA) of dissected muscles were measured. Relative SOD activity and expression of MHC and phosphorylated Akt, ERK were measured after dissection. RESULTS: Muscle weight and Type I, II fiber CSA of hindlimb muscles in the CDDP group were significantly less than the control group. Muscle weight and Type I, II fiber CSA of hindlimb muscles, food intake, activity, and withdrawal thresholds of the CDDP-SOD group were significantly greater than the CDDP group. There were no significant differences in relative SOD activities of hindlimb muscles between the CDDP-SOD and CDDP groups. MHC expression and phosphorylated Akt, ERK of hindlimb muscles in the CDDP-SOD group were significantly greater than the CDDP group. CONCLUSION: Cu/Zn SOD attenuates hindlimb muscular atrophy induced by cisplatin through increased food intake and activity. Increment of phosphorylated Akt, ERK may relate to attenuation of hindlimb muscular atrophy.
