The effects of blastocyst morphological score and blastocoele re-expansion speed after warming on pregnancy outcomes.
10.5653/cerm.2016.43.1.31
- Author:
Huiqun YIN
1
;
Hong JIANG
;
Ruibing HE
;
Cunli WANG
;
Jie ZHU
;
Yang LI
Author Information
1. Reproductive Medicine Center, 105th Hospital of PLA, Hefei, China. jiangh105@sina.com
- Publication Type:Original Article
- Keywords:
Blastocyst;
Embryo transfer;
Embryonic development;
Freezing;
Pregnancy outcome
- MeSH:
Blastocyst*;
Cardiopulmonary Resuscitation;
Cryopreservation;
Embryo Transfer;
Embryonic Development;
Female;
Freezing;
Humans;
Logistic Models;
Pregnancy;
Pregnancy Outcome*;
Pregnancy Rate;
Pregnancy*;
Retrospective Studies
- From:Clinical and Experimental Reproductive Medicine
2016;43(1):31-37
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study was to investigate associations between the morphology score of blastocysts and blastocoele re-expansion speed after warming with clinical outcomes, which could assist in making correct and cost-effective decisions regarding the appropriate time to vitrify blastocysts and to transfer vitrified-warmed blastocysts. METHODS: A total of 327 vitrified-warmed two-blastocyst transfer cycles in women 38 years old and younger were included in this retrospective study. RESULTS: The clinical pregnancy rate (CPR) and implantation rate (IR) of transfers of two good-morphology grade 4 blastocysts vitrified on day 5 (64.1% and 46.8%, respectively) were significantly higher than the CPR and IR associated with the transfers of two good-morphology grade 3 blastocysts vitrified on day 5 (46.7% and 32.2%, respectively). No significant differences were found in the CPR and IR among the transfers of two good-morphology grade 4 blastocysts regardless of the day of cryopreservation. Logistic regression analysis showed that blastocoele re-expansion speed after warming was associated with the CPR. CONCLUSION: The selection of a good-morphology grade 4 blastocyst to be vitrified could be superior to the choice of a grade 3 blastocyst. Extending the culture of grade 3 blastocysts and freezing grade 4 or higher blastocysts on day 6 could lead to a greater likelihood of pregnancy. Since re-expansion was shown to be a morphological marker of superior blastocyst viability, blastocysts that quickly re-expand after warming should be prioritized for transfer.
