Co-Culture of alpha TC-6 Cells and beta TC-1 Cells: Morphology and Function.
- Author:
Sung Man KIM
1
;
Eun Ju LEE
;
Hye Sook JUNG
;
Na HAN
;
You Jeong KIM
;
Tae Kyoon KIM
;
Tae Nyun KIM
;
Min Jeong KWON
;
Soon Hee LEE
;
Jeong Hyun PARK
;
Byoung Doo RHEE
;
Mi Kyung KIM
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: alpha-Cell; beta-Cell; Insulin
- MeSH: Cell Culture Techniques; Coculture Techniques*; Endocrine Cells; Glucagon; Glucose; Immunoassay; Insulin; Islets of Langerhans
- From:Endocrinology and Metabolism 2015;30(1):92-97
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: In vitro experiments using only beta-cell lines instead of islets are limited because pancreatic islets are composed of four different types of endocrine cells. Several recent studies have focused on cellular interactions among these cell types, especially alpha- and beta-cells. Because islet isolation needs time and experience, we tested a simple co-culture system with alpha- and beta-cells. Their morphology and function were assessed by comparison to each single cell culture and pancreatic islets. METHODS: alpha TC-6 cells and beta TC-1 cells were maintained in Dulbecco's Minimal Essential Medium containing 5 mM glucose and 10% fetal bovine serum. Cells were mixed at a 1:1 ratio (5x10(5)) in 6-well plates and cultured for 24, 48, and 72 hours. After culture, cells were used for insulin and glucagon immunoassays and tested for glucose-stimulated insulin secretion (GSIS). RESULTS: alpha TC-6 and beta TC-1 cells became condensed by 24 hours and were more strongly compacted after 48 hours. beta TC-1 cells showed both beta-beta and beta-alpha cell contacts. GSIS increased with increasing glucose concentration in co-cultured cells, which showed lower secreted insulin levels than beta TC-1 cells alone. The increase in the secreted insulin/insulin content ratio was significantly lower for co-cultured cells than for beta-cells alone (P=0.04). Compared to islets, the alpha-/beta-cell co-culture showed a higher ratio of GSIS to insulin content, but the difference was not statistically significant (P=0.09). CONCLUSION: alpha TC-6 and beta TC-1 cells in the co-culture system showed cell-to-cell contacts and a similar stimulated insulin secretion pattern to islets. The co-culture system may be used to better mimic pancreatic islets in in vitro assessments.
