Blockade of vascular angiogenesis by Aspergillus usamii var. shirousamii-transformed Angelicae Gigantis Radix and Zizyphus jujuba.
- Author:
Sang Wook KANG
1
;
Jung Suk CHOI
;
Ji Young BAE
;
Jing LI
;
Dong Shoo KIM
;
Jung Lye KIM
;
Seung Yong SHIN
;
Hyun Ju YOU
;
Hyoung Sook PARK
;
Geun Eog JI
;
Young Hee KANG
Author Information
- Publication Type:Original Article
- Keywords: Angiogenesis; Aspergillus. usamii var. shirousamii; transformation; Angelicae Gigantis Radix and Zizyphus jujuba
- MeSH: Angelica; Aspergillus; Basement Membrane; Endothelial Cells; Extracellular Matrix; Human Umbilical Vein Endothelial Cells; Hyperplasia; Matrix Metalloproteinases; Neoplasm Metastasis; Phorbols; Plants, Medicinal; Transendothelial and Transepithelial Migration; Ziziphus
- From:Nutrition Research and Practice 2009;3(1):3-8
- CountryRepublic of Korea
- Language:English
- Abstract: The matrix metalloproteinases (MMP) play an important role in tumor invasion, angiogenesis and inflammatory tissue destruction. Increased expression of MMP was observed in benign tissue hyperplasia and in atherosclerotic lesions. Invasive cancer cells utilize MMP to degrade the extracellular matrix and vascular basement membrane during metastasis, where MMP-2 has been implicated in the development and dissemination of malignancies. The present study attempted to examine the antiangiogenic activity of the medicinal herbs of Aspergillus usamii var. shirousamii-transformed Angelicae Gigantis Radix and Zizyphus jujube (tAgR and tZj) with respect to MMP-2 production and endothelial motility in phorbol 12-myristate 13-acetate (PMA)- or VEGF-exposed human umbilical vein endothelial cells (HUVEC). Nontoxic tAgR and tZj substantially suppressed PMA-induced MMP-2 secretion. In addition, 25 microg/mL tAgR and tZj prevented vascular endothelial growth factor-stimulated endothelial cell transmigration and tube formation. The results reveal that tAgR and tZj dampened endothelial MMP-2 production leading to endothelial transmigration and tube formation. tAgR and tZj-mediated inhibition of endothelial MMP may boost a therapeutic efficacy during vascular angiogenesis.
