Mucosal CD8+ T Cell-Mediated Immunity of Vaccinized Animals to Genital Infection of Herpes Simplex Virus Type 1.
- Author:
Seong Kug EO
1
;
Hyun A YOON
;
John Hwa LEE
;
Joon Seok CHAE
;
Jeong Gon CHO
Author Information
1. Department of Microbiology, College of Veterinary Medicine and Bio-Safety Institute of Chonbuk National University, Jeonju 561-756, Korea. vetvirus@chonbuk.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Mucosal CD8+ T cells;
Herpes simplex virus;
DNA vaccine;
CTL assay;
MHC tetramer staining
- MeSH:
Animals*;
Cell Count;
DNA;
Herpes Simplex*;
Herpesvirus 1, Human*;
Immunity, Cellular*;
Lymph Nodes;
Lymphocytes;
Mice;
Simplexvirus*;
T-Lymphocytes
- From:Journal of Bacteriology and Virology
2004;34(1):39-46
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, we directly evaluated mucosal CD8+ T cell-mediated immunity using ex vivo cytotoxic T lymphocyte (CTL) assay and MHC class I tetramer staining method in iliac lymph node (LN) and vaginal tracts of mice immunized mucosally with several prime-boost protocols after genital infection of herpes simplex virus type 1 (HSV-1). Ex vivo CTL activity in iliac LN of infected mice was evaluated at 3-day post-infection without in vitro 5-day stimulation. Iliac LN of mice immunized with recombinant viral vaccine-priming and DNA vaccine-boosting protocol showed more potent CTL activity than those of other groups. Such ex vivo CTL activity was consistent with mucosal gB498-505 (SSIEFARL)-specific CD8+ T cell number of vaginal tract determined by MHC class I (H-2b) tetramer containing immunodominant peptide. Furthermore, the number of mucosal SSIEFARL-specific CD8+ T cells recruited into infected genital tracts appeared to decide the protective outcome against genital infection of virulent HSV-1. These results support that mucosal CD8+ T cells are principal mediators for the protection against genital infection of HSV-1.
