Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii.
10.3347/kjp.2005.43.1.27
- Author:
Aifen LIN
;
Eun Hee SHIN
;
Tae Yun KIM
;
Jae Hwan PARK
;
Sang Mee GUK
;
Jong Yil CHAI
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii;
Korean isolate-1 (KI-1);
PCR-RFLP;
sequence
- MeSH:
Amino Acid Sequence;
Animals;
Antigens, Protozoan/*genetics;
Base Sequence;
Genes, Protozoan;
Genotype;
Polymorphism, Genetic;
RNA, Protozoan;
Research Support, Non-U.S. Gov't;
Toxoplasma/*genetics
- From:The Korean Journal of Parasitology
2005;43(1):27-32
- CountryRepublic of Korea
- Language:English
-
Abstract:
Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5' and 3' ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.
