Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease.
10.3347/kjp.2005.43.1.33
- Author:
Myeong Heon SHIN
;
Young Bae CHUNG
;
Hirohito KITA
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Paragonimus westermani;
excretory-secretory products (ESP);
cysteine protease;
eosinophils;
degranulation
- MeSH:
Animals;
*Cell Degranulation;
Cysteine Endopeptidases/metabolism/*physiology;
Eosinophil-Derived Neurotoxin/metabolism;
Eosinophils/*physiology;
Humans;
Paragonimus westermani/*enzymology;
Research Support, Non-U.S. Gov't;
Superoxides/metabolism;
Time Factors
- From:The Korean Journal of Parasitology
2005;43(1):33-37
- CountryRepublic of Korea
- Language:English
-
Abstract:
Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EDN) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.
