Immunoglobulin Gene Repertoire In Rheumatoid Synovium As A Model For Autoimmune Disease.
- Author:
Soo Kon LEE
1
;
Joon Hyun SONG
Author Information
1. Department of Internal Medicine, Yonsei University College of Medicine Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Immunoglobulin gene;
Repertoire;
Rheumatoid arthritis;
Somatic mutation;
CDR 3 lengths
- MeSH:
Arthritis, Rheumatoid;
Autoimmune Diseases*;
Complementarity Determining Regions;
DNA, Complementary;
Genes, Immunoglobulin*;
Humans;
Immunoglobulin kappa-Chains;
Immunoglobulins*;
Lymphocytes;
Synovial Membrane*
- From:The Journal of the Korean Rheumatism Association
1994;1(2):200-212
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To gain insights into structural characteristics of immunoglobulin kappa chain repertoire expressed in the inflammed synovium of rheumatoid arthritis (RA), we analyzed V kappa transcirpts expressed in the synovium of a patient with longstanding RA and compared to those expressed in the PBLs of RA and normal controls. METHODS: RT-PCR was done to amplify kappa chain transcripts from RA synovial lymphocytes and the cDNA sequences were compared to those from PBL of RA patient or normal control. RESULTS: Kappa chain repertoire from RA patient's synovial lymphocytes or PBL revealed increased somatic mutation and unusually long complementarity determining region (CDR) 3 compared to normal control. CONCLUSIONS: These changes in kappa chain repertoire in RA patient are suggesting that the antibody repertoire expressed in the synovium or PBL is unique and may be related with systemic dysregulation of B cell development
