Effect of exogenous gonadotropin on HB-EGF expression in endometrium.
- Author:
Seung Yup KU
1
;
Byung Chul JEE
;
Chang Suk SUH
;
Seok Hyun KIM
;
Young Min CHOI
;
Jung Gu KIM
;
Jin Yong LEE
;
Shin Yong MOON
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Medical Research Center, Seoul National University, Korea. ymchoi@snu.ac.kr
- Publication Type:Original Article ; In Vitro
- Keywords:
HB-EGF;
Endometrium;
Mouse;
Ovarian hyperstimulation
- MeSH:
Animals;
Embryo Transfer;
Endometrium*;
Female;
Fertilization in Vitro;
Gonadotropins*;
Mice;
Pregnancy;
RNA, Messenger;
Uterus
- From:Korean Journal of Obstetrics and Gynecology
2005;48(5):1265-1270
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Gonadotropin hyperstimulation for in vitro fertilization and embryo transfer (IVF-ET) has been considered to be harmful to the uterine receptivity. The role of HB-EGF has been implicated in the mouse embryonic implantation. In the present study, we assessed the effect of gonadotropin hyperstimulation on the expression of HB-EGF mRNA in murine endometrium. METHODS: Competitive, reverse transcription-PCR was performed to evaluate the expression of HB-EGF mRNA in the hyperstimulated murine endometrum from pregnant uteri of gestation day (g.d.) 5.5 after the injection of saline (placebo), pregnant mare serum gonadotropin (PMSG) 5 IU and 10 IU. RESULTS: HB-EGF expression was observed at 10-4-10-6 dilution concentrations of internal standard, and dual bands (423/360 bp) were observed at 10-5 dilution concentration. The expression of HB-EGF mRNA showed no significant change in the endometrial expression in PMSG groups, compared to the control group, on g.d. 5.5. There was no dose-response relationship between the two PMSG groups. CONCLUSION: Our data reveals that ovarian hyperstimulation using gonadotropin does not influence the expression of HB-EGF in the murine endometrium. Further studies are necessary to elucidate which stage of embryonic implantation and the expression of other substance is affected by the ovarian hyperstimulation.
