Stable Hepatocyte Engraftment after Hepatocyte Transplantation Using Biodegradable Injectable Polymer.
- Author:
Dong Ho CHOI
1
;
Eun ROH
;
Han Joon KIM
;
Kyeong Geun LEE
;
Seung Sam PAIK
;
Sang Hun LEE
;
Yong Sung LEE
;
Doo Jin PAIK
;
Byung Soo KIM
;
Kwang Soo LEE
Author Information
1. Department of Surgery, College of Medicine, Hanyang University, Korea. kslee@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Hepatocyte transplantation;
Biodegradable injectable polymer
- MeSH:
Animals;
Cell Survival;
Cryopreservation;
Female;
Hepatocytes*;
Humans;
Immunohistochemistry;
Liver;
Liver Transplantation;
Male;
Mice;
Mice, Nude;
Microspheres;
Peritoneal Cavity;
Polymers*;
Tissue Donors;
Trypan Blue
- From:The Journal of the Korean Society for Transplantation
2002;16(2):233-237
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Whole liver transplantation, an effective therapy for many inherited and acquired hepatic disorders, has limitations including donor shortage and fatal surgical complications. Hepatocyte transplantation, which is simpler and less expensive than whole liver transplantation, allows the use of living related donors, permits the use of a single donor organ for multiple recipients, and makes possible the cryopreservation of hepatocytes for future use. However, choosing a proper scaffold for hepatocytes hampers wide use of hepatocyte transplantation. We performed hepatocyte transplantation using biodegradable injectable polymers, fabricated form poly (lactide-co-glycolide) (PLGA) microspheres, as scaffolds to evaluate their effectiveness. METHODS: Female, five week old FVB mice, were prepared for donors, and four male, five week old nude mice, were used for recipients. Liver cells were isolated from FVB donors. The cell viability exceeded 95% as assessed by trypan blue exclusion method. For three nude mice, 2X10(6) cells resuspended in 200micro liter medium were mixed with 200micro liter PLGA microspheres, and were injected into the peritoneal cavity of each mouse. One nude mouse was transplanted with 2X10(6) cells resuspended in 200micro liter medium only, and it served as a negative control. Specimens were retrieved at one week, and histological and immunohistochemical analyses were performed. RESULTS: In the negative control, all transplanted hepatocytes disappeared at one week. In mice transplanted both microspheres and hepatocytes, conglomerates, which contained hepatocytes, were observed in the peritoneal cavity, The hepatocytes were identified by H and E staining and immunohistochemistry using anti- hepatocyte antibody. CONCLUSION: In this preliminary study, stable hepatocyte engraftment was achieved in hepatocyte transplantation with PLGA microspheres, but not in hepatocyte transplantation only. More studies on comparison between sponge-type scaffolds and injectable scaffolds would be necessary. Improvement on both initial vascularization and proliferation of transplanted hepatocytes is a target of our future work.
