Immunostimulatory effects of BCG-CWS on the proliferation and viability of mouse spleen cells.
- Author:
Che Wook LEE
1
;
Eun Ju KO
;
Hong Gu JOO
Author Information
1. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. jooh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
adjuvant;
BCG-CWS;
spleen cell;
vaccine
- MeSH:
Animals;
Bacillus;
Cell Proliferation;
Collodion;
Diminazene;
Immunotherapy;
Interferon-gamma;
Interleukin-2;
Lymphocytes;
Mice;
Mycobacterium bovis;
Skeleton;
Spleen;
Trypan Blue
- From:Korean Journal of Veterinary Research
2012;52(2):89-97
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been tried to use for immunotherapy. The CWS of Mycobacterium bovis bacillus Calmette-Guerin (BCG-CWS) was studied as an universal vaccine vehicle for antigen conjugation, to develop potentially effective and safe vaccine. Although a variety of biological activities of BCG-CWS have been studied, the effects of BCG-CWS on spleen cells are not fully elucidated. Using MTT assay and trypan blue exclusion test, we found that BCG-CWS significantly enhanced the viability and proliferation of cells. Multiple clusters, indicating proliferation, were observed in BCG-CWS-treated spleen cells and surface marker staining assay revealed that BCG-CWS promoted the proliferation of CD19+ B lymphocyte rather than CD4+ or CD8+ T lymphocyte. In addition, BCG-CWS up-regulated the expression of anti-apoptotic molecules such as bcl-2, bcl-xL. BCG-CWS increased the surface expression of CD25 and CD69 as well as IL-2 production of spleen cells, suggesting increased activation. Furthermore, BCG-CWS enhanced the antigen-specific cell proliferation and interferon-gamma production of spleen cells. Taken together, these results demonstrate the immunostimulatory effects of BCG-CWS on spleen cells via multiple mechanisms, providing valuable information to broaden the use of BCG-CWS in clinical and research settings.
