In vitro and in vivo antibacterial activity of Meliae fructus extract against Helicobacter pylori.
- Author:
Hyun A LEE
1
;
Okjin KIM
Author Information
1. Center for Animal Resources Development, Wonkwang University, Iksan 570-749, Korea. kimoj@wku.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
antibacterial activity;
anti-helicobacter;
Helicobacter pylori;
herb;
Meliae fructus
- MeSH:
Animals;
Ethanol;
Gerbillinae;
Helicobacter;
Helicobacter pylori;
Humans;
Mass Screening;
Melia;
Plants;
Plants, Medicinal;
Stomach Ulcer;
Urease
- From:Korean Journal of Veterinary Research
2012;52(2):105-113
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In this study, a medicinal herbal plant, Meliae fructus, was examined and screened for anti-Helicobacter (H.) pylori activity. Seventy percent ethanol was used for herbal extraction. For anti-H. pylori activity screening, inhibitory zone tests as an in vitro assay and in vivo study using a Mongolian gerbil (Meriones unguiculatus) model were performed. Also, the safety of herbal compounds was evaluated by animal study. As a result of inhibitory zone test, Meliae fructus extract demonstrated strong anti-H. pylori activities. Also, as results of in vivo animal studies, Meliae fructus demonstrated strong therapeutic effects against H. pylori infection according to the criteria of histological examination and rapid urease test. As results of the safety study, after 28 days treatment of the Meliae fructus extract, the animals were not detected any grossly and histological changes. These results demonstrate that it can be successfully cured against H. pylori infection and protected from H. pylori-induced pathology with Meliae fructus. It could be a promising native herbal treatment for patients with gastric complaints including gastric ulcer caused by H. pylori.
