Sequential pathologic changes and viral distribution in rabbits experimentally infected with new Korean strain of rabbit hemorrhagic disease virus (RHDVa).
- Author:
Jung Won PARK
1
;
Ji Eun CHUN
;
Dong Kun YANG
;
Eun Jung BAK
;
Han KIM
;
Myeong Heon LEE
;
Eui Kyung HWANG
;
Chung Bok LEE
;
Gye Hyeong WOO
Author Information
1. Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-757, Korea.
- Publication Type:Original Article
- Keywords:
chronic infection;
histopathologic changes;
new RHDVa serotype;
rabbit hemorrhagic disease;
viral distribution
- MeSH:
Dental Caries;
Hemorrhagic Disease Virus, Rabbit;
Kidney;
Korea;
Liver;
Lung;
Necrosis;
Polymerase Chain Reaction;
Rabbits;
Spleen;
Sprains and Strains;
Ureter;
Uterus;
Virus Diseases
- From:Korean Journal of Veterinary Research
2012;52(2):125-131
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.
