DNA-mediated immunization of mice with plasmid encoding HBs antigen.
10.3346/jkms.1999.14.2.187
- Author:
Soo Jung YOON
1
;
Jong Wook PARK
;
Su Yul AHN
;
Byung Kil CHOE
;
Min Ho SUH
Author Information
1. Department of Immunology, School of Medicine, and Institute for Medical Science, Keimyung University, Taegu, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Vaccines;
DNA;
Hepatitis B surface antigens
- MeSH:
Animal;
Cloning, Molecular;
DNA, Viral/immunology*;
Hepatitis B/prevention & control*;
Hepatitis B Antibodies/immunology;
Hepatitis B Surface Antigens/genetics*;
Hepatitis B Vaccines/immunology*;
Human;
Interleukin-2/biosynthesis;
Male;
Mice;
Mice, Inbred ICR;
Plasmids/immunology*;
Spleen/immunology;
Spleen/cytology;
Vaccination;
Vaccines, DNA/immunology*
- From:Journal of Korean Medical Science
1999;14(2):187-192
- CountryRepublic of Korea
- Language:English
-
Abstract:
In order to develop an experimental DNA vaccine for the prevention and treatment of hepatitis B virus infection, hepatitis B virus surface antigen (HBsAg) DNA was subcloned into an E. coli-eukaryotic cell shuttle vector and was expressed in the Baculovirus expression system. Intramuscular, intradermal, and intraperitoneal injections of 30 microg of the plasmid DNA expressing HBsAg induced humoral and cellular immune responses in ICR mice. The first IgG antibodies were detected after ten days and specific IgG antibody titers peaked after two months of a single intramuscular DNA injection. Anti-HBs antibody titers gradually increased and peaked at four months following intradermal DNA injection, and in case of intraperitoneal injection they peaked at seven months. Generation of HBs-specific helper T lymphocytes was also investigated through the production of interleukin-2 by T helper cells. Boosting effects of HBs DNA were investigated without much results. In general, DNA-mediated HBs immunization induced humoral and cellular immune responses in mice that appears to simulate immune responses in human during the course of HBV vaccination.
