Effects of Peroxynitrite on HSP 70 Expression in Cultured Normal Human Nasal Epithelial Cell.
- Author:
Tae Young JANG
1
;
Yoon Seok CHOI
;
Kyu Sung KIM
;
Kook Jin KO
;
Tae Man KIM
Author Information
1. Department of Otorhinolaryngology-Head & Neck Surgery, Inha University College of Medicine, Incheon, Korea. jangty@inha.ac.kr
- Publication Type:Original Article
- Keywords:
Nitric oxide;
Peroxynitrite;
Heat-shock proteins 70
- MeSH:
Endothelium-Dependent Relaxing Factors;
Epithelial Cells*;
HSP70 Heat-Shock Proteins;
Humans*;
Iron;
Metabolism;
Nasal Mucosa;
Nitric Oxide;
Peroxynitrous Acid*;
Physiological Processes;
Rhinitis;
RNA, Messenger;
Sinusitis;
Synaptic Transmission;
Tissue Donors;
Vasodilation
- From:Journal of Rhinology
2005;12(2):88-91
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Nitric Oxide (NO) is an endogenous mediator first characterized as an endothelium-derived relaxing factor. It is now recognized as a key mediator in many physiological process such as vasodilatation, neurotransmission, host defense, and iron metabolism. However, much remains to be determined about the pathophysiological role of NO in the airway. Peroxynitrite, which is synthesized by NO, is the diret cause of cellular toxicity in inflammatory reaction. In this study, we investigated the cellular toxcity of peroxynitrite by the expression of Heat-shock proteins 70 (HSP 70) in normal human nasal epithelium (NHNE) at the inflammatory conditions MATERIALS AND METHODS: 3-Morpholinosydronimone clorhydrate which is a peroxynitrite donor was mixed in the media of cultured NHNE cell. RESULTS: HSP 70 was expressed at the peroxynitrite environment of cultured NHNE cells and HSP 70 mRNA was detected with a time-dependent increasing pattern. CONCLUSION: Peroxynitrite may have a cytotoxic effect, and inhibition of peroxynitrite synthesis may have an important role for controlling the cytotoxic and inflammatory conditions of rhinitis and sinusitis.
