15-Lipoxygenase-1 Mediates Mucociliary Differentiation in Normal Human Nasal Epithelial Cells.
- Author:
Jung Hyun CHANG
1
;
Jeong Hong KIM
;
Joo Heon YOON
;
Jeung Gweon LEE
;
Hyung Seok SEO
;
Kyung Su KIM
Author Information
1. Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea. ydrhinol@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Arachidonate, 15-Lipoxygenase;
Nasal mucosa;
Cell differentiation;
Cilia
- MeSH:
Blotting, Western;
Cell Differentiation;
Cilia;
Culture Media;
Epithelial Cells*;
Epithelium;
Gene Expression;
Humans*;
Interleukin-4;
Microscopy, Electron, Scanning;
Nasal Mucosa;
Tretinoin
- From:Journal of Rhinology
2005;12(2):92-96
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: 15-lipoxygenase-1 (15-LO-1) is involved in the differentiation of human tracheobronchial epithelial cells. Here we investigated the relation between 15-LO-1 expression and the differentiation of normal human nasal epithelial (NHNE) cells. MATERIALS AND METHODS: NHNE cells, RT-PCR, Western blot analysis, and scanning electron microscopy (SEM) were used. RESULTS: In retinoic acid (RA)-sufficient culture media, 15-LO-1 expression in NHNE cells increased time-dependently, but its expression was undetectable in RA-deficient culture media. Moreover, in RA-deficient culture media, IL-4 time-dependently induced 15-LO-1 expression at a concentration of 1 ng/mL. In addition, MUC8 gene expression, a marker of mucociliary differentiation, was up-regulated by 15-LO-1, which was itself induced by IL-4. In SEM, the ciliated epithelium was observed with the treatment of IL-4. CONCLUSION: Our findings suggest that 15-LO-1 may be related to the differentiation of human nasal epithelium, and that it may mediate the mucociliary differentiation of NHNE cells.
